- Description
- From GEO summary: Genome binding/occupancy profiling by high throughput sequencing. DNA duplication is intimately connected to setting up post-replicative chromosome structures and events, but molecular details of this coordination are not well understood. A striking example occurs during yeast meiosis, where replication locally influences timing of the DNA double-strand breaks (DSBs) that initiate recombination. We show here that replication-DSB coordination is eliminated by overexpressing Dbf4-dependent Cdc7 kinase (DDK) or removing Tof1 or Csm3, components of the replication fork protection complex (FPC). DDK physically associates with Tof1, and Tof1 is dispensable for replication-DSB coordination if DDK is artificially tethered to replisomes. Furthermore, DDK phosphorylation of the DSB-promoting factor Mer2 is locally coordinated with replication, dependent on Tof1. These findings indicate that DDK recruited by FPC to replisomes phosphorylates chromatin-bound Mer2 in the wake of the replication fork, thus synchronizing replication with an early prerequisite for DSB formation. This may be a general mechanism to ensure spatial and temporal coordination of replication with other chromosomal processes.
-
Access via GEO
Plain Text Sequencing Data
Accession #: GSE52970Access via SRAChIP Sequence reads for 96 samples
Accession #: SRP033495Access via BioProjectAdditional information about overall initiative.
Accession #: PRJNA230576 - Access Restrictions
-
Free to All
- Access Instructions
- The NCBI Gene Expression Omnibus database provides open access to these files.
- Associated Publications
- Data Type
- Equipment Used
- Dataset Format(s)
- Plain Text, SRA, TAR, gzip
- Dataset Size
- 3.0 Gb (Plain Text), 70.24 Gb (SRA)
Do you have or know of a dataset that should be added to the catalog? Let us know!