Spo11-oligo mapping in S. cerevisiae red1, hop1, mek1 mutants
UID: 10501
Core Facilities: Bioinformatics, Integrated Genomics Operation (IGO)- Description
- From GEO summary: Genome binding/occupancy profiling by high throughput sequencing. Meiotic recombination starts with the formation of DNA double-strand breaks (DSBs) made by Spo11. In Saccharomyces cerevisiae, the nonrandom distribution of meiotic DSBs along the genome can be attributed to the combined influence of multiple factors on Spo11 cleavage. One factor is higher-order chromatin structure, particularly the loop-axis organization of meiotic chromosomes. Axial element proteins Red1 and Hop1 provide the basis for meiotic loop-axis organization and are implicated in diverse aspects of meiotic recombination. Mek1 is a meiotic-specific kinase associated with Red1 and Hop1. Red1, Hop1, and Mek1 are required for normal DSB levels, but their effects on the DSB distribution has not been examined, and exactly how these proteins influence DSB levels and distribution is unknown. Here, we examined the contributions of Red1, Hop1, and Mek1 to the DSB distribution by deep sequencing and mapping Spo11-associated oligonucleotides from red1, hop1, and mek1 mutant strains, thereby generating genome-wide meiotic DSB maps.
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Access via GEO
TAR of WIG Sequencing Data.
Accession #: GSE84859Access via SRAOTHER Sequencing data for 6 samples.
Accession #: SRP079912Access via BioProjectAdditional information about overall initiative.
Accession #: PRJNA335382 - Access Restrictions
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Free to All
- Access Instructions
- The NCBI Gene Expression Omnibus database provides open access to these files.
- Associated Publications
- Data Type
- Equipment Used
- Dataset Format(s)
- SRA, TAR, WIG
- Dataset Size
- 12.3 MB (TAR of WIG), 2.6 Gb (SRA)
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