- Description
- From GEO summary: Genome binding/occupancy profiling by high throughput sequencing. To segregate accurately during meiosis, homologous chromosomes in most species must recombine. Very small chromosomes would risk missegregation if recombination were randomly distributed, so the double-strand breaks (DSBs) that initiate recombination are not haphazard. How this nonrandomness is controlled is not understood. Here we demonstrate that Saccharomyces cerevisiae integrates multiple, temporally distinct pathways to regulate chromosomal binding of pro-DSB factors Rec114 and Mer2, thereby controlling duration of a DSB-competent state. Homologous chromosome engagement regulates Rec114/Mer2 dissociation late in prophase, whereas replication timing and proximity to centromeres or telomeres influence timing and amount of Rec114/Mer2 accumulation early. A distinct early mechanism boosts Rec114/Mer2 binding quickly to high levels specifically on the shortest chromosomes, dependent on chromosome axis proteins and subject to selection pressure to maintain hyperrecombinogenic properties of these chromosomes. Thus, an organism’s karyotype and its attendant risk of meiotic missegregation influence the shape and evolution of its recombination landscape.
-
Access via GEO
Accession #: GSE119786Access via SRAChIP Sequence reads for 24 samples.
Accession #: SRP161479Access via BioProjectAdditional information about overall initiative.
Accession #: PRJNA490317 - Access Restrictions
-
Free to All
- Access Instructions
- The NCBI Gene Expression Omnibus database provides open access to these files.
- Associated Publications
- Data Type
- Equipment Used
- Dataset Format(s)
- Plain Text, SRA, gzip
- Dataset Size
- 960.9 Mb (TXT), 19.3 Gb (SRA)
Do you have or know of a dataset that should be added to the catalog? Let us know!