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scRNAseq transcriptome analysis of developing regulatory T cells and precursors in the thymus.

UID: 10517

Author(s): Hemmers, Saskia*, Schizas, Michail*, Azizi, Elham*, Dikiy, Stanislav*, Zhong, Yi*, Feng, Yongqiang*, Gregoire Altan-Bonnet*, Rudensky, Alexander Y.* * MSK affiliated

Description
Summary from the GEO: "We analyzed the individual transcriptomes of thymic Treg cells (CD4+Foxp3+), their immediate precursors (CD25+CD4+Foxp3-) and mature CD4 single positive thymocytes (CD4+Foxp3-CD25-CD62L+CD24-).
To gain insight into characteristic features of thymocytes producing IL-2 in situ, single-cell RNA sequencing data was collected from mature CD25-Foxp3-CD4SP, CD25+Foxp3- and Foxp3+ CD4SP thymocytes."
Subject of Study
Mus musculus
Subject(s)
Thymocytes
Thymus Gland
Thymus Neoplasms
Transcriptome
OncoTree Cancer Type(s)
Thymus
Access via GEO

RNA sequencing data.
Accession #: GSE134902

Access via SRA

RNA Sequence reads for 3 samples.
Accession #: SRP216429

Access via BioProject

Additional information about overall initiative.
Accession #: PRJNA556716

Access Restrictions
Free to All
Access Instructions
The NCBI Gene Expression Omnibus database provides open access to these files. SRA and/or tar files can be downloaded directly from the site or viewed in the NCBI SRA Run Selector (link at bottom of page).
Associated Publications
Hemmers S, Schizas M, Azizi E, et al. IL-2 production by self-reactive CD4 thymocytes scales regulatory T cell generation in the thymus. Journal of Experimental Medicine. 2019; 216(11):2466-2478.
Data Type
Genomic
Equipment Used
Illumina NovaSeq 6000
Dataset Format(s)
CSV, SRA, TAR
Dataset Size
25.6 Mb (TAR of CSV), 14.3 Gb (SRA).
Data Catalog Record Updated
2023-12-07