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Cryo-EM structure of Csm-crRNA-target RNA ternary complex in complex with AMPPNP in type III-A CRISPR-Cas system

UID: 10518

Author(s): Jia, Ning*, Patel, Dinshaw J.* * MSK affiliated

Description
This deposit in the Research Collaboratory for Structural Bioinformatics Protein Data Base (PDB) includes cryogenic electron microscopy data identifying the structure of Csm-crRNA-target RNA ternary complex in complex with AMPPNP in type III-A CRISPR-Cas system. The main summary display for this entry includes an experimental data snapshot, protein source, macromolecules and small molecules included in the deposit, and version history and funding identification. Data is provided for download as FASTA sequence format, the Protein Data Bases own PDB format, crystallographic information file (.cif), XML, the EM map, as well as the full validation file in either PDF or XML formats. Additionally, the PDB provides embedded visualization software to display a 3D view of the protein: PDB's Mol* viewer, NGL viewer, and JSmol. PDB provides protein family and gene product annotations, additional details about the experiment parameters, a Protein Feature Viewer (in the Sequence tab) which displays all available features and alignments of known polymer instances, and a genome view which provides graphical summaries of the correspondences between PDB Entity sequences and genomes.
Subject of Study
Subject(s)
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Research Collaboratory for Structural Bioinformatics Protein Data Base
Accession #: 6O7E

Access Restrictions
Free to All
Access Instructions
The RCSB Protein Data Base provides access to 3D structure data for large biological molecules (proteins, DNA, and RNA). Sequence, protein structure, EM, and other data can be downloaded in formats specific to the data they contain.
DOI
10.2210/pdb6O7E/pdb
Associated Publications
Data Type
Software Used
JSmol
Mol*
NGL
Dataset Format(s)
PDF, XML, FASTA, PDB, CIF, MAP
Dataset Size
1.47 MB (PDB Format)
Data Catalog Record Updated
2021-04-15