Effect of Ro 08-2750 treatment on gene expression of human leukemia cell lines MOLM13 and K562
UID: 10539
- Description
- Summary from the GEO: "RNA-sequencing on human leukemia cell lines MOLM13 (AML, MLL-AF9+) and K562 (CML-BC, BCR-ABL+) after Ro 08-2750 treatment (20 uM, 4hours).
Cells were cultured at 0.5M/mL and treated with the small-molecule Ro 08-2750 for 4hours at 20 uM."
The dataset is associated with the MUSASHI (MSI) family of RNA binding proteins (MSI1 and MSI2). According to the associated article, the MUSASHI family contributes "...to a wide spectrum of cancers including acute myeloid leukemia. We find that the small molecule Ro 08–2750 (Ro) binds directly and selectively to MSI2 and competes for its RNA binding in biochemical assays. Ro treatment in mouse and human myeloid leukemia cells results in an increase in differentiation and apoptosis, inhibition of known MSI-targets, and a shared global gene expression signature similar to shRNA depletion of MSI2. Ro demonstrates in vivo inhibition of c-MYC and reduces disease burden in a murine AML leukemia model." A small molecule that targets MSI’s oncogenic activity was identified and the associated study study provides a framework for targeting RNA binding proteins in cancer. .
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Access via GEO
CSV and GZIP sequencing data.
Accession #: GSE114320Access via SRARNA Sequence reads for 16 samples.
Accession #: SRP145352Access via BioProjectAdditional information about overall initiative.
Accession #: PRJNA470944 - Access Restrictions
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Free to All
- Access Instructions
- The NCBI Gene Expression Omnibus, SRA, and BioProject databases provide open access to these files.
- Associated Publications
- Data Type
- Equipment Used
- Dataset Format(s)
- CSV, SRA, gzip
- Dataset Size
- 645.0 Kb (CSV), SRA broken up into 16 files of 117.3Mb - 1.8Gb
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