Cryo-EM structure of Csm-crRNA binary complex in type III-A CRISPR-Cas system

UID: 10578

Author(s): Jia, Ning*, Wang, Chongyuan*, Eng, Edward T., Patel, Dinshaw J.* * MSK affiliated

Description: This deposit in the Research Collaboratory for Structural Bioinformatics Protein Data Base (PDB) includes cryogenic electron microscopy data identifying the Cryo-EM structure of Csm-crRNA binary complex in type III-A CRISPR-Cas system. The main summary display for this entry includes information on the experimental method, protein source, macromolecules and small molecules included in the deposit, and version history and funding identification. Data is provided for download as FASTA sequence format, the Protein Data Bases own PDB format, crystallographic information file (.cif), XML, the EM map, as well as the full validation file in either PDF or XML formats. Additionally, the PDB provides embedded visualization software to display a 3D view of the protein: PDB's Mol* viewer, NGL viewer, and JSmol. PDB provides protein family and gene product annotations, additional details about the experiment parameters, a Protein Feature Viewer (in the Sequence tab) which displays all available features and alignments of known polymer instances, and a genome view which provides graphical summaries of the correspondences between PDB Entity sequences and genomes.

Access to this dataset is also provided by the Electron Microscopy Data Bank (EMDB).

Subject of Study

Subject(s)

Access via PDB
Research Collaboratory for Structural Bioinformatics Protein Data Base
Accession #: 6MUU

Access via EMDB
Research Collaboratory for Structural Bioinformatics Protein Data Base and Electron Microscopy Data Bank
Accession #: 9256
Access Restrictions: Free to All
Access Instructions: The RCSB Protein Data Base provides access to 3D structure data for large biological molecules (proteins, DNA, and RNA). Sequence, protein structure, EM, and other data can be downloaded in formats specific to the data they contain.
DOI: 10.2210/pdb6MUU/pdb
Associated Publications: Jia N, Mo C, Wang C, et al. Type III-A CRISPR-Cas Csm complexes: Assembly, periodic RNA cleavage, DNase activity regulation, and autoimmunity. Molecular Cell. 2019; 73(2):264-277.e5.
Data Type: Analytical Chemistry
Software Used: JSmol
HTML5 modality of the Jmol open-source Java viewer for chemical structures in 3D

Mol*
Plugin created by the Protein Data Base to visualize protein structures

NGL
NGL Viewer is a web application for molecular visualization.
Dataset Format(s): PDF, XML, FASTA, PDB, CIF, MAP
Dataset Size: 1,407 Kb (PDB Format)

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