Inter-myeloid cell synaptic antigen dispersal drives diversified anti-tumor T cell priming

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UID: 10597

Author(s): Roberts, Edward, Krummel, Matthew F.

Description: Summary from the GEO: "Using a labelled tumor antigen we identify dendritic cell subsets containing tumor derived antigen in the tumor draining lymph nodes and used these to stimulate anti-tumor OTI CD8 T cells. These were subsequently interrogated by RNAseq to determine how diverse DC subsets drive distinct patterns of CD8 T cell differentiation"

Overall design from the GEO: "OTI CD8 T cell RNAseq analysis on cells stimulated in vitro by different DC subsets"

Subject of Study

Subject(s)

Access via GEO
XLSX file of expression profiling by high throughput sequencing
Accession #: GSE128980

Access via SRA
RNA Sequence reads for 8 samples
Accession #: SRP189732

Access via BioProject
Additional information about overall initiative.
Accession #: PRJNA529639
Access Restrictions: Free to All
Access Instructions: The NCBI Gene Expression Omnibus, SRA, and BioProject databases provide open access to these files.
Associated Publications: Ruhland MK, Roberts EW, Cai E, et al. Visualizing Synaptic Transfer of Tumor Antigens among Dendritic Cells. Cancer Cell. 2020;37(6):786-799.e5.
Data Type: Genomic
Equipment Used: Illumina HiSeq 2000
High throughput gene sequencing system
Software Used: SRA Toolkit
The NCBI SRA Toolkit enables reading ("dumping") of sequencing files from the SRA database and writing ("loading") files into the .sra format.
Dataset Format(s): SRA, XLSX
Dataset Size: 14 MB (XLSX), 6.3 Gb (SRA)

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