Search Tips

Expression data from thymic endothelial cells after damage

UID: 10623

Author(s): Wertheimer, Tobias*, Velardi, Enrico*, Cooper, Kirsten, Tsai, Jennifer*, Dudakov, Jarrod Andrew*, Van Den Brink, Marcel R. M.* * MSK affiliated

Description
Summary from the GEO: "The thymus is extremely sensitive to damage but also has a remarkable ability to repair itself. However, the mechanisms underlying this endogenous regeneration remain poorly understood and this capacity diminishes considerably with age. To identify alternate regeneration pathways in the thymus, we performed an unbiased transcriptome analysis of the non-hematopoietic (CD45-) stromal cell compartment of the thymus, which is less sensitive to thymic damage compared to the CD45+ hematopoietic compartment.
Recent work has suggested that ECs can contribute towards thymic regeneration via their production of so-called angiocrine factors. Using a technique to constitutively activate the Akt pathway in ECs using the pro-survival adenoviral gene E4ORF1, ECs can be propagated and expanded ex vivo (exEC) while maintaining their phenotype and vascular tube formation capacity. We compared the gene expression of freshly isolated thymic ECs and exEC-derived from the thymus.
Thymic non-hematopoietic stromal cells were isolated using CD45 MACS cell depletion. Endothelial cells were then FACS purified (CD45- EpCAM- VE-Cad+). exEC were generated by lentiviral transduction with the E4ORF1 gene. Microarray analysis was performed on an Affymetrix MOE 430 A 2.0 platform in triplicate for freshly isolated ECs from untreated mice, and for exECs transduced with the pro-survival adenoviral gene E4ORF1."
Subject of Study
Mus musculus
Subject(s)
Endothelial Cells
Thymus Gland
Thymus Neoplasms
OncoTree Cancer Type(s)
Thymus
Access via GEO

Raw and CEL sequencing data.
Accession #: GSE106980

Access via BioProject

Additional information about the overall initiative.
Accession #: PRJNA418719

Access Restrictions
Free to All
Access Instructions
The NCBI Gene Expression Omnibus, SRA, and BioProject databases provide open access to these files.
Data Type
Genomic
Equipment Used
Affymetrix Mouse Genome 430A 2.0 Array
Software Used
GEO2R

Use GEO2R to compare two or more groups of Samples in order to identify genes that are differentially expressed across experimental conditions. Results are presented as a table of genes ordered by significance.

Dataset Format(s)
TAR, CEL, RAW
Dataset Size
7.1 Mb (TAR of CEL)
Data Catalog Record Updated
2021-10-12