MeRIP-seq mapping of m6A in the IFN-β induced transcriptome in Huh7 cells
UID: 10671
- Description
- Summary from the GEO: "The RNA modification N6-methyladenosine (m6A) regulates gene expression through various transcript-specific effects. The goal of this experiment was to identify m6A-modified transcripts during the type I interferon response, with interest in determining which interferon-stimulated genes are modified by m6A. We found that a high percentage of ISGs are m6A-modified, and in follow-up experiments we determined the effects of m6A on the expression of interferon-stimulated genes. We found that m6A regulates the translation of a subset of ISGs, which in turn augments the antiviral effects of the type I interferon respnose."
Overall design from the GEO: "Huh7 cells were seeded in 15-cm plates and treated with IFN-β at 50 units/mL or mock treated for 8 hours prior to harvest and MeRIP-seq was performed. 3 biological replicates were performed for each condition."
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Access via GEO
Plain Text Data of meRIP (m6A) sequencing data
Accession #: GSE155413Access via SRARIP Sequence reads for 12 samples
Accession #: SRP274283Access via BioProjectAdditional information about overall initiative.
Accession #: PRJNA649691 - Access Restrictions
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Free to All
- Access Instructions
- The NCBI Gene Expression Omnibus, SRA, and BioProject databases provide open access to these files.
- Associated Publications
- Data Type
- Equipment Used
- Software Used
- Dataset Format(s)
- Plain Text, SRA
- Data Tool(s)
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meRIP Seq
- Dataset Size
- 1.8 MB (TXT), 36.8 Gb (SRA)
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