Shared and distinct metabolic demands for innate and adaptive cytotoxic lymphocytes during viral infection

UID: 10685

Author(s): Santosa, Endi K*, Sun, Joseph C.* * MSK affiliated

Publisher(s): Memorial Sloan Kettering Cancer Center

Description: Summary from GEO: "Natural Killer (NK) cells and CD8+ T cells share many characteristics at steady state and during viral infection. In this study, we interrogate the metabolic pathways that fuel NK cell effector function in response to mouse cytomegalovirus (MCMV) infection. This dataset contains transcriptional profiling of wild-type and LDHA-deficient Ly49Hpositive NK cells at steady state and early after infection."

Overall design from GEO: "RNASeq was performed on wild-type or LDHA-deficient Ly49Hpositive NK cells harvested after 2 days post in vivo infection in a mixed bone chimera setting; 2-3 replicates per group and 20,000-60,000 cells per replicate."

Subject of Study

Subject(s)

Access via GEO
Plain Text Data of expression profiling by high throughput sequencing
Accession #: GSE149447

Access via SRA
RNA Sequence reads for 11 samples
Accession #: SRP258736

Access via BioProject
Additional information about overall initiative.
Accession #: PRJNA628693
Access Restrictions: Free to All
Access Instructions: The NCBI Gene Expression Omnibus, SRA, and BioProject databases provide open access to these files.
Associated Publications: Sheppard S, Santosa E, Lau C, et al. Lactate dehydrogenase A-dependent aerobic glycolysis promotes natural killer cell anti-viral and anti-tumor function. Cell Reports. 2021; 35(9):109210.
Data Type: Genomic
Equipment Used: Illumina HiSeq 4000
High throughput gene sequencing system
Software Used: SRA Toolkit
The NCBI SRA Toolkit enables reading ("dumping") of sequencing files from the SRA database and writing ("loading") files into the .sra format.
Dataset Format(s): Plain Text, SRA, gzip
Data Tool(s): RNA Seq
Dataset Size: 373.1 KB (Plain Text), 15.9 Gb (SRA)

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