Dysfunction and stemness of tumor-infiltrating T cells are triggered by a common mechanism
UID: 10720
Publisher(s): United States - National Institutes of Health (NIH) - National Cancer Institute (NCI)- Description
- Summary from the GEO: "The paradox of tumor immunology is that tumor infiltrating lymphocytes (TILs) are dysfunctional in situ and yet are capable of stem cell-like behavior with self-renewal, massive expansion, multipotency, and eradication of large metastatic tumors. Here we report that the overabundance of potassium in the tumor microenvironment (TME) can explain both phenomena."
Overall design from the GEO: "Ctrl (or Samples labelled V- Vehicle) and elevated potassium (or Samples labelled K) were used to perfrom ChiP assays. CD8+ T cells from pmel mice were stimulated in vitro with 1 μM human gp100 peptide (10 μg/ml) in Ctrl (V) or elevated potassium (K) for 5 days and secondary re-stimulation was done for 48hrs with plate-bound anti-CD3 (1 μg/ml; BD Biosciences) and soluble anti-CD28 (1 μg/ml; BD Biosciences) and expanded in culture medium (Ctrl /K) containing 60 IU/mL of IL-2 for a total of 10 days."
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Access via GEO
BED and BIGWIG files of genome binding/occupancy profiling by high throughput sequencing
Accession #: GSE122156Access via SRAChIP Sequencing data from 10 samples
Accession #: SRP167854Access via BioProjectAdditional information about the overall inititative.
Accession #: PRJNA503845 - Access Restrictions
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Free to All
- Access Instructions
- The NCBI Gene Expression Omnibus, SRA and BioProject databases provide open access to these files.
- Associated Publications
- Data Type
- Equipment Used
- Software Used
- Dataset Format(s)
- BED, BIGWIG
- Data Tool(s)
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ChIP Seq
- Dataset Size
- 533.7 Kb (BED), 1,810.1 Kb (BIGWIG)
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