DNAJC9 Integrates Heat Shock Molecular Chaperones into the Histone Chaperone Network

UID: 10734

Author(s): Hammond, Colin M., Reverón-Gómez, Nazaret, Groth, Anja

Description: Summary from the GEO: "From biosynthesis to assembly into nucleosomes, histones are handed through a cascade of histone chaperones, which shield histones from non-specific interactions. Whether mechanisms exist to safeguard the histone fold during histone chaperone handover events or to release trapped intermediates is unclear. Using structure-guided and functional proteomics, we identify and characterize a histone chaperone function of DNAJC9, a heat shock co-chaperone that promotes HSP70-mediated catalysis. We elucidate the structure of DNAJC9, in a histone H3-H4 co-chaperone complex with MCM2, revealing how this dual histone and heat shock co-chaperone binds histone substrates. We show that DNAJC9 recruits HSP70-type enzymes via its J domain to fold histone H3-H4 substrates: upstream in the histone supply chain, during replication- and transcription-coupled nucleosome assembly, and to clean up spurious interactions. With its dual functionality, DNAJC9 integrates ATP-resourced protein folding into the histone supply pathway to resolve aberrant intermediates throughout the dynamic lives of histones."

Overall design from the GEO: "Quantitative ChIP-seq analysis of DNAJC9 distribution and abundance from cells expressing DNAJC9-MYC-Flag WT, J or 4A mutants, and control cells . All experiments were done in duplicates. Chromatin of Drosophila melanogater S2 cells were used as spike-in for normalization purpose."

Subject of Study

Subject(s)

Access via GEO
BEDGRAPH files of genome binding/occupancy profiling by high throughput sequencing
Accession #: GSE154445

Access via SRA
ChIP Sequencing data of 9 samples
Accession #: SRP271863

Access via BioProject
Additional information about the overall inititative.
Accession #: PRJNA646272
Access Restrictions: Free to All
Access Instructions: The NCBI Gene Expression Omnibus, SRA and BioProject databases provide open access to these files. SRA and/or tar files can be downloaded directly from the site or viewed in the NCBI SRA Run Selector (link at bottom of page).
Associated Publications: Hammond C, Bao H, Hendriks I, et al. DNAJC9 integrates heat shock molecular chaperones into the histone chaperone network. Molecular Cell. 2021; 81(12):2533-2548.e9.
Data Type: Genomic
Equipment Used: Illumina NextSeq 500
Software Used: SRA Toolkit
The NCBI SRA Toolkit enables reading ("dumping") of sequencing files from the SRA database and writing ("loading") files into the .sra format.
Dataset Format(s): SRA, TAR, BEDGRAPH
Data Tool(s): ChIP Seq
Dataset Size: 51.8 MB (TAR of BEDGRAPH), 3.2 Gb (SRA)

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