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Crystal structure of DNAJC9 HBD in complex with H3.3-H4 dimer and MCM2 HBD

UID: 10736

Author(s): Bao, Hongyu, Huang, Hongda* * MSK affiliated

Description
This deposit in the Research Collaboratory for Structural Bioinformatics Protein Data Base (PDB) includes electron microscopy data identifying the crystal structure of DNAJC9 HBD in complex with H3.3-H4 dimer and MCM2 HBD. The main summary display for this entry includes information on the experimental method, validation, macromolecules and small molecules included in the deposit, as well as version history and funding identification. Data is provided for download as FASTA sequence format, the Protein Data Bases own PDB format, crystallographic information file (.cif), XML, the EM map, as well as the full validation file in either PDF or XML formats. Additionally, the PDB provides embedded visualization software to display a 3D view of the protein: PDB's Mol* viewer, NGL viewer, and JSmol. PDB provides protein family and gene product annotations, additional details about the experiment parameters, a Protein Feature Viewer (in the Sequence tab) which displays all available features and alignments of known polymer instances, and a genome view which provides graphical summaries of the correspondences between PDB Entity sequences and genomes.
Subject of Study
Homo sapiens
Subject(s)
Histone Chaperones
Histones
HSP40 Heat-Shock Proteins
Minichromosome Maintenance Complex Component 2
Access via PDB

Research Collaboratory for Structural Bioinformatics Protein Data Base
Accession #: 7CJ0

Access Restrictions
Free to All
Access Instructions
The RCSB Protein Data Base provides access to 3D structure data for large biological molecules (proteins, DNA, and RNA). Sequence, protein structure, EM, and other data can be downloaded in formats specific to the data they contain.
DOI
10.2210/pdb7CJ0/pdb
Associated Publications
Hammond C, Bao H, Hendriks I, et al. DNAJC9 integrates heat shock molecular chaperones into the histone chaperone network. Molecular Cell. 2021; 81(12):2533-2548.e9.
Data Type
Analytical Chemistry
Equipment Used
DECTRIS EIGER X 16M

X-ray detector with optimized solid-state sensors and CMOS readout ASICs in hybrid pixel technology

SSRF (Shanghai Synchrotron Radiation Facility) Beamline BL17U1

Used for high resolution and high throughput structural determination of macromolecules and their complexes

Software Used
JSmol

HTML5 modality of the Jmol open-source Java viewer for chemical structures in 3D

Mol*

Plugin created by the Protein Data Base to visualize protein structures

NGL

NGL Viewer is a web application for molecular visualization.

PHASER

Phaser is a program for phasing macromolecular crystal structures with maximum likelihood methods.

PHENIX

A software suite for the automated determination of molecular structures using X-ray crystallography and other methods

xia2

System to perform X-ray diffraction data processing

Dataset Format(s)
PDF, XML, FASTA, PDB, CIF, MTZ, DSN6
Data Tool(s)
X-ray crystallography (XRC)
Dataset Size
643 KB (FASTA) 206 KB (PDB)
Data Catalog Record Updated
2021-07-15