Listeria seeligeri LS46 and LS59 Genome sequencing and assembly

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UID: 10744

Publisher(s): Rockafeller University

Description: BioProject description: "Genome sequencing of Listeria seeligeri LS46 and LS59".

The whole genome sequencing of these listeriaphage enabled researchers to discover that AcrVIA1, an anti-CRISPR protein, interacts with the guide-exposed face of Cas13a, a (crRNA)-guided nuclease, preventing access to the target RNA and the conformational changes required for nuclease activation. A single dose of AcrVIA1 delivered by an individual virion completely dismantles type VI-A CRISPR-mediated immunity.

Subject of Study

Subject(s)

Access via BioProject
Links experiments and whole genome and RNA sequencing results
Accession #: PRJNA607241
Access Restrictions: Free to All
Access Instructions: The NCBI BioPrjoect database provides open access to the SRA Experiments and Nucleotide genome sequencing data. SRA and/or tar files can be downloaded directly from the site or viewed in the NCBI SRA Run Selector.
Associated Publications: Meeske A, Jia N, Cassel A, et al. A phage-encoded anti-CRISPR enables complete evasion of type VI-A CRISPR-Cas immunity. Science. 2020; 369(6499):54-59.
Data Type: Genomic
Equipment Used: Illumina NextSeq 500
Software Used: SRA Toolkit
The NCBI SRA Toolkit enables reading ("dumping") of sequencing files from the SRA database and writing ("loading") files into the .sra format.
Dataset Format(s): FASTA
Data Tool(s): RNA Seq

WGS
Dataset Size: 1.86 GB

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