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Cryo-EM structure of Cas13(crRNA)

UID: 10745

Author(s): Jia, Ning*, Meeske, Alexander J., Marraffini, Luciano A., Patel, Dinshaw J.* * MSK affiliated

Description
This deposit in the Research Collaboratory for Structural Bioinformatics Protein Data Base (PDB) includes electron microscopy data used for modeling the Cryo-EM structure of Cas13(crRNA). The main summary display for this entry includes information on the experimental data, validation, macromolecules and version history. Data is provided for download as FASTA sequence format, the Protein Data Bases own PDB format, crystallographic information file (.cif), XML, DSN6 Maps, MTZ Map Coefficients, as well as the full validation file in either PDF or XML formats. Additionally, the PDB provides embedded visualization software to display a 3D view of the protein: PDB's Mol* viewer, NGL viewer, and JSmol. PDB provides protein family and gene product annotations, additional details about the experiment parameters, a Protein Feature Viewer (in the Sequence tab) which displays all available features and alignments of known polymer instances, and a genome view which provides graphical summaries of the correspondences between PDB Entity sequences and genomes.
Subject of Study
Listeria seeligeri
Subject(s)
Clustered Regularly Interspaced Short Palindromic Repeats
CRISPR-Associated Proteins
CRISPR-Cas Systems
DNA-Directed RNA Polymerases
Listeria
Access via PDB

Research Collaboratory for Structural Bioinformatics Protein Data Base
Accession #: 6VRC

Access Restrictions
Free to All
Access Instructions
The RCSB Protein Data Base provides access to 3D structure data for large biological molecules (proteins, DNA, and RNA). Sequence, protein structure, EM, and other data can be downloaded in formats specific to the data they contain.
DOI
10.2210/pdb6VRC/pdb
Associated Publications
Meeske A, Jia N, Cassel A, et al. A phage-encoded anti-CRISPR enables complete evasion of type VI-A CRISPR-Cas immunity. Science. 2020; 369(6499):54-59.
Data Type
Analytical Chemistry
Equipment Used
FEI TITAN KRIOS

Electron microscope and imaging robot optimized for high-resolution structural biology.

GATAN K3

Single electron counting direct detection camera

Software Used
JSmol

HTML5 modality of the Jmol open-source Java viewer for chemical structures in 3D

Mol*

Plugin created by the Protein Data Base to visualize protein structures

NGL

NGL Viewer is a web application for molecular visualization.

RELION

RELION (for REgularised LIkelihood OptimisatioN) is a stand-alone computer program for Maximum A Posteriori refinement of (multiple) 3D reconstructions or 2D class averages in cryo-electron microscopy. It is developed in the research group of Sjors Scheres at the MRC Laboratory of Molecular Biology.

Dataset Format(s)
PDF, XML, FASTA, PDB, CIF, MAP
Data Tool(s)
Electron Microscopy
Dataset Size
174 KB (PDB)
Data Catalog Record Updated
2021-07-22