Description: This deposit in the Research Collaboratory for Structural Bioinformatics Protein Data Base (PDB) includes x-ray diffraction data identifying the crystal structure of human REV7(R124A)-SHLD3(35-58) complex. The main summary display for this entry includes information on the experimental method, validation, macromolecules in the deposit, as well as entry history. Data is provided for download as FASTA sequence format, the Protein Data Bases own PDB format, crystallographic information file (.cif), XML, the EM map, as well as the full validation file in either PDF or XML formats. Additionally, the PDB provides embedded visualization software to display a 3D view of the protein: PDB's Mol* viewer, NGL viewer, and JSmol. PDB provides protein family and gene product annotations, additional details about the experiment parameters, a Protein Feature Viewer (in the Sequence tab) which displays all available features and alignments of known polymer instances, and a genome view which provides graphical summaries of the correspondences between PDB Entity sequences and genomes.

Subject of Study

Homo sapiens

Subject(s)

DNA-Binding Proteins

DNA-Directed RNA Polymerases

Mad2 Proteins

Nuclear Proteins

Access via PDB
prote
Accession #: 6WW9
Access Restrictions: Free to All
Access Instructions: The RCSB Protein Data Base provides access to 3D structure data for large biological molecules (proteins, DNA, and RNA). Sequence, protein structure, EM, and other data can be downloaded in formats specific to the data they contain.
DOI: 10.2210/pdb6WW9/pdb
Associated Publications: Xie W, Wang S, Wang J, et al. Molecular mechanisms of assembly and TRIP13-mediated remodeling of the human Shieldin complex. Proceedings of the National Academy of Sciences of the United States of America. 2021; 118(8):e2024512118.
Data Type: Analytical Chemistry
Equipment Used: APS (Advanced Photon Source) Beamline 24-ID-C
Sector 24 utilizes canted insertion devices to provide two independent x-ray sources, one for a variable energy beamline 24-ID-C and another for a fixed energy beamline 24-ID-E (Se peak). Both beamlines are optimized to address challenging cases in macromolecular crystallography such as large complex molecular assemblies, poorly diffracting crystals and microcrystals by providing extremely stable and intense x-ray beams with beam sizes ranging from 5 to 70 microns. The 24-ID-C beamline is equipped with an ALS-style sample auto-mount robotic system for increased productivity and a mini-kappa goniometer head for crystal alignment.

DECTRIS PILATUS3 S 6M
Hybrid Photon Counting X-ray detector
Software Used: JSmol
HTML5 modality of the Jmol open-source Java viewer for chemical structures in 3D

Mol*
Plugin created by the Protein Data Base to visualize protein structures

NGL
NGL Viewer is a web application for molecular visualization.

PHENIX
A software suite for the automated determination of molecular structures using X-ray crystallography and other methods

XDS
X-ray Detector Software for processing single-crystal monochromatic diffraction data recorded by the rotation method.
Dataset Format(s): PDF, XML, FASTA, PDB, CIF, MTZ, DSN6
Data Tool(s): X-ray crystallography (XRC)
Dataset Size: 429 KB (FASTA), 76 KB (PDB)

Data Catalog Record Updated: 2021-07-26

Crystal structure of human REV7(R124A)-SHLD3(35-58) complex