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RNA-seq on parental and acquired resistant cells (AqR)

UID: 10763

Description
Summary from the GEO: "Parental and AqR cells were obtained at diferent time-points (Day1 and Day 18). Total RNA was extracted and submitted for RNA-seq. Differential expression was observed between parental and AqR cells. AqR cells were derived from parental cells by adding inhibitor (CAY10566) every 3 days until cells became resistant (at about 3 weeks)."

Overall Design from the GEO: "2 parental cell lines in duplicate (G68 D1 A,B and G82 D1 A,B) and 2 AqR cell lines in duplicate (G68 D18 A,B and G82 D18 A,B) were submitted and analyzed."

This dataset contains the RNA sequencing from glioblastoma patient tumor samples for which there was a low median expression of the lipogenic enzyme stearoyl CoA desaturase (SCD). Cell lines from this subset of patients expressed undetectable SCD, yet retained residual SCD enzymatic activity. These lines evolved to survive independent of SCD through unknown mechanisms. Cell lines that escaped such genetic and epigenetic alterations expressed higher levels of SCD and were highly dependent on SCD for survival.
Subject of Study
Subject(s)
OncoTree Cancer Type(s)
CNS/Brain
Glioblastoma
Access via GEO

XLSX file of RNA Sequencing
Accession #: GSE131005

Access via SRA

RNA sequencing of 8 samples.
Accession #: SRP197533

Access via BioProject

Additional information about the overall inititative.
Accession #: PRJNA542273

Access Restrictions
Free to All
Access Instructions
The NCBI Gene Expression Omnibus, SRA and BioProject databases provide open access to these files. SRA and/or tar files can be downloaded directly from the site or viewed in the NCBI SRA Run Selector (link at bottom of page).
Associated Publications
Data Type
Equipment Used
Illumina HiSeq 2500
Software Used
SRA Toolkit
Dataset Format(s)
Microsoft Excel
Data Tool(s)
RNA Seq
Dataset Size
67.1 MB
Data Catalog Record Updated
2023-12-07