ATAC-seq on parental and acquired resistant cells (AqR)
- Summary from GEO: "Parental and AqR cells were obtained. DNA was purified and submited for sequencing. Differential chromatin accessiblity was observed. AqR cells were derived from parental cells by adding inhibitor (CAY10566) every 3 days until cells became resistant (at about 3 weeks)."
Overall design: from GEO: "1 parental cell in duplicate and 1 AqR cell in duplicate was submitted and analyzed."
This dataset contains the ATAC sequencing from glioblastoma patient tumor samples for which there was a low median expression of the lipogenic enzyme stearoyl CoA desaturase (SCD). Cell lines from this subset of patients expressed undetectable SCD, yet retained residual SCD enzymatic activity. These lines evolved to survive independent of SCD through unknown mechanisms. Cell lines that escaped such genetic and epigenetic alterations expressed higher levels of SCD and were highly dependent on SCD for survival.