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UAMS Smoldering Myeloma Timeline Cohort

UID: 10784

Publisher(s): UAMS (University of Arkansas for Medical Sciences) Myeloma Center

Description
Dataset Description from the EGA: "To better understand the pattern of genetic changes over time, we performed whole exome sequencing of sequential bone marrow samples from 9 patients taken overtime including some paired SMM/newly diagnosed MM/Relapse MM samples.
Samples from 9 patients (9 controls and 53 tumors) underwent whole exome sequencing with an additional capture for the IGH, IHK, IGL, and MYC loci. DNA was obtained from either CD138+ cells from the bone marrow of smoldering myeloma patients through time (tumor) or from stem cell harvests or peripheral blood cells from the same patient (control). 100 ng of DNA was fragmented, end-repaired, and adapters ligated using NimbleGen's MedExome. After PCR amplification hybridized libraries underwent further amplification before being sequenced on a NextSeq500 (Illumina) using 75 bp paired end reads. "
Subject of Study
Homo sapiens
Subject(s)
Multiple Myeloma
Neoplasms, Plasma Cell
Oncogene Proteins, Fusion
Smoldering Multiple Myeloma
OncoTree Cancer Type(s)
Myeloid
Access via EGA

BAM files of 62 samples
Accession #: EGAD00001005056

Access Restrictions
Application Required
Access Instructions
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Associated Publications
Boyle E, Deshpande S, Tytarenko R, et al. The molecular make up of smoldering myeloma highlights the evolutionary pathways leading to multiple myeloma. Nature Communications. 2021; 12:293.
Data Type
Genomic
Equipment Used
Illumina NextSeq 500
Dataset Format(s)
BAM
Data Tool(s)
WXS
Targeted DNA Seq
Dataset Size
520 GB
Data Catalog Record Updated
2021-07-28