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Cryo-EM structure of CARD8-CARD filament

UID: 10789

Description
This deposit in the Research Collaboratory for Structural Bioinformatics Protein Data Base (PDB) includes cryogenic electron microscopy data identifying the cryo-EM structure of CARD8-CARD filament. The main summary display for this entry includes information on the experimental method, validation and macromolecules in the deposit, as well as entry history and funding information. Data is provided for download as FASTA sequence format, the Protein Data Bases own PDB format, crystallographic information file (.cif), XML, the EM map, as well as the full validation file in either PDF or XML formats. Additionally, the PDB provides embedded visualization software to display a 3D view of the protein: PDB's Mol* viewer, NGL viewer, and JSmol. PDB provides protein family and gene product annotations, additional details about the experiment parameters, a Protein Feature Viewer (in the Sequence tab) which displays all available features and alignments of known polymer instances, and a genome view which provides graphical summaries of the correspondences between PDB Entity sequences and genomes.

The associated EMPIAR Image Set, linked here, includes the 2D tiff images use to develop the 3D modeling.
Subject of Study
Subject(s)
Access via PDB

Research Collaboratory for Structural Bioinformatics Protein Data Base
Accession #: 6XKJ

Access via EMPIAR

Multi-frame micrographs for the CARD8 CT filament
Accession #: EMPIAR-10567

Access Restrictions
Free to All
Access Instructions
The RCSB Protein Data Base provides access to 3D structure data for large biological molecules (proteins, DNA, and RNA). Sequence, protein structure, EM, and other data can be downloaded in formats specific to the data they contain.

EMPIAR, the Electron Microscopy Public Image Archive, is a public resource for raw electron microscopy images, the raw images used to build a 3D structure. Image sets are available for browsing and download. Accessions are linked to their related PDB and EMDB entries.
DOI
10.2210/pdb6XKJ/pdb
Associated Publications
Data Type
Equipment Used
FEI TITAN KRIOS
FEI VITROBOT MARK III
Software Used
CTFFIND
JSmol
Mol*
NGL
PHENIX
REFMAC5
RELION
Serial EM
Dataset Format(s)
PDF, XML, FASTA, PDB, CIF, MAP
Data Tool(s)
Electron Microscopy
Dataset Size
218 KB (FASTA), 199 KB (PDB)
Data Catalog Record Updated
2021-08-03