TMEM41B is a pan-flavivirus host factor
UID: 10819
- Description
- Summary from the GEO: "Flaviviruses pose a constant threat to human health. These RNA viruses are transmitted by the bite of infected mosquitoes and ticks and regularly cause outbreaks. To identify host factors required for flavivirus infection we performed full-genome loss of function CRISPR-Cas9 screens. Based on these results we focused our efforts on characterizing the roles that TMEM41B and VMP1 play in the virus replication cycle. Our mechanistic studies on TMEM41B revealed that all members of the Flaviviridae family that we tested require TMEM41B. We tested 12 additional virus families and found that SARS-CoV-2 of the Coronaviridae also required TMEM41B for infection. Remarkably, single nucleotide polymorphisms (SNPs) present at nearly twenty percent in East Asian populations reduce flavivirus infection. Based on our mechanistic studies we propose that TMEM41B is recruited to flavivirus RNA replication complexes to facilitate membrane curvature, which creates a protected environment for viral genome replication."
Overall design from the GEO: "Genome-scale CRISPR screens targeting were performed for ZIKV (PRVABC59 strain) and YFV (Asibi strain)."
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Access via GEO
Plain text file
Accession #: GSE162040Access via SRASequencing of the 40 samples from the experiement
Accession #: SRP293864Access via BioProjectAdditional information about the overall inititative.
Accession #: PRJNA680347 - Access Restrictions
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Free to All
- Access Instructions
- The NCBI Gene Expression Omnibus, SRA, and BioProject databases provide open access to these files. The SRA Run Selector link at the bottom of the page is a processing tool for raw data.
- Associated Publications
- Data Type
- Equipment Used
- Software Used
- Dataset Format(s)
- Plain Text, SRA
- Dataset Size
- 2 MB (TXT), 3.6 Gb (SRA)
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