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Cryo-EM structure of the CRAC channel Orai in an open conformation; H206A gain-of-function mutation in complex with an antibody

UID: 10828

Author(s): Long, Stephen Barstow*, Hou, Xiaowei*, Outhwaite, Ian R.* * MSK affiliated

Description
This deposit in the Research Collaboratory for Structural Bioinformatics Protein Data Base (PDB) includes cryogenic electron microscopy data identifying the cryo-EM structure of the CRAC channel Orai in an open conformation; H206A gain-of-function mutation in complex with an antibody. The main summary display for this entry includes information on the experimental method, validation, macromolecules and small molecules in the deposit, as well as entry history and funding information. Data is provided for download as FASTA sequence format, the Protein Data Bases own PDB format, crystallographic information file (.cif), XML, the EM map, as well as the full validation file in either PDF or XML formats. Additionally, the PDB provides embedded visualization software to display a 3D view of the protein: PDB's Mol* viewer, NGL viewer, and JSmol. PDB provides protein family and gene product annotations, additional details about the experiment parameters, a Protein Feature Viewer (in the Sequence tab) which displays all available features and alignments of known polymer instances, and a genome view which provides graphical summaries of the correspondences between PDB Entity sequences and genomes.
Subject of Study
Drosophila melanogaster
Mus musculus
Subject(s)
Calcium Release Activated Calcium Channels
Carrier Proteins
ORAI1 Protein
Access via PDB

Research Collaboratory for Structural Bioinformatics Protein Data Base
Accession #: 7KR5

Access Restrictions
Free to All
Access Instructions
The RCSB Protein Data Base provides access to 3D structure data for large biological molecules (proteins, DNA, and RNA). Sequence, protein structure, EM, and other data can be downloaded in formats specific to the data they contain.
DOI
10.2210/pdb7KR5/pdb
Associated Publications
Hou X, Outhwaite I, Pedi L, Long S. Cryo-EM structure of the calcium release-activated calcium channel Orai in an open conformation. eLife. 2020; 9:e62772.
Data Type
Analytical Chemistry
Equipment Used
cryoSPARC

software platform for cryo-electron microscopy

FEI TITAN KRIOS

Electron microscope and imaging robot optimized for high-resolution structural biology.

GATAN K2 SUMMIT

14.2 Megapixel camera optimized for single particle structural biology, cryo-tomography, plus high-speed dynamic and in-situ electron microscopy.

Software Used
cryoSPARC

Software platform for cryo-electron microscopy

CTFFIND

Program for finding CTFs of electron micrographs

JSmol

HTML5 modality of the Jmol open-source Java viewer for chemical structures in 3D

Mol*

Plugin created by the Protein Data Base to visualize protein structures

NGL

NGL Viewer is a web application for molecular visualization.

RELION

RELION (for REgularised LIkelihood OptimisatioN) is a stand-alone computer program for Maximum A Posteriori refinement of (multiple) 3D reconstructions or 2D class averages in cryo-electron microscopy. It is developed in the research group of Sjors Scheres at the MRC Laboratory of Molecular Biology.

Dataset Format(s)
PDF, XML, FASTA, PDB, CIF, MAP
Data Tool(s)
Electron Microscopy
Dataset Size
986 KB (FASTA), 201 KB (PDB)
Data Catalog Record Updated
2021-08-11