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Description
Summary from the GEO: "We demonstrate that Msi2 is the predominant form expressed in hematopoietic stem cells (HSC), and its knockdown leads to reduced engraftment and depletion of HSCs in vivo. Overexpression of Msi2 in a mouse model increases HSC cell cycle progression and cooperates with BCR-ABL1 to induce an aggressive leukemia. MSI2 is over-expressed in human myeloid leukemia, and expression levels directly correlate with decreased patient survival, thereby defining MSI2 expression as a novel prognostic marker in acute myeloid leukemia (AML). Depletion of MSI2 in human myeloid leukemia cells leads to decreased proliferation and apoptosis. These data implicate the MSI2 RNA binding protein in myeloid leukemogenesis and identify a novel potential target for therapy in AML.
We transduced four human leukemia cell lines with lentiviral shRNA vectors targeting MSI2. Hybridizations of treated and control samples from each cell line were dye swap replicated."
Subject of Study
Homo sapiens
Subject(s)
Leukemia, Myeloid, Acute
Access via GEO

RAW and Plain Text Sequencing Data
Accession #: GSE22775

Access via BioProject

Additional information about overall initiative.
Accession #: PRJNA129145

Access Restrictions
Free to All
Access Instructions
The NCBI Gene Expression Omnibus, SRA, and BioProject databases provide open access to these files.
Associated Publications
Nguyen D, Lu Y, Chu K, et al. HyperTRIBE uncovers increased MUSASHI-2 RNA binding activity and differential regulation in leukemic stem cells. Nature Communications. 2020; 11:2026.
Data Type
Genomic
Equipment Used
Agilent-014850 Whole Human Genome Microarray 4x44K G4112F (Feature Number version)
Dataset Format(s)
Plain Text, TAR, RAW
Dataset Size
132.5 Mb (TAR of TXT)
Data Catalog Record Updated
2021-08-11