Gene expression from normal and Msi2 KO mouse LSK cells

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UID: 10831

Author(s): Kharas, Michael G.* * MSK affiliated

Description: Summary from the GEO: "Hematopoietic stem and progenitor cells (Lineagelo ScaI+ c-Kit+) were sorted 4 weeks post pIpC injection. RNA was extracted using TRIZOL and RNEASY RNA extraction kit. RNA was then amplified using NUGEN Pico amplification kit, fragmented and hybridized on Mouse Expression Array 430 2.0. Signal normalization was performed by RMA method. Data were analyzed using GSEA across the complete list of genes ranked by signal-to-noise ratio.
Samples came from bone marrow of 4 normal mice and 4 mice with conditional Msi2 knockout."

Subject of Study

Subject(s)

Access via GEO
TAR and CEL Sequencing Data
Accession #: GSE53385

Access via BioProject
Additional information about overall initiative.
Accession #: PRJNA232066
Access Restrictions: Free to All
Access Instructions: The NCBI Gene Expression Omnibus, SRA, and BioProject databases provide open access to these files.
Associated Publications: Nguyen D, Lu Y, Chu K, et al. HyperTRIBE uncovers increased MUSASHI-2 RNA binding activity and differential regulation in leukemic stem cells. Nature Communications. 2020; 11:2026.
Data Type: Genomic
Equipment Used: Affymetrix Mouse Genome 430 2.0 Array
Software Used: GEO2R
Use GEO2R to compare two or more groups of Samples in order to identify genes that are differentially expressed across experimental conditions. Results are presented as a table of genes ordered by significance.
Dataset Format(s): TAR, CEL, RAW
Dataset Size: 27.2 Mb (TAR of CEL)

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