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Data from: Forward genetic screen of human transposase genomic rearrangements

UID: 10851

Author(s): Henssen, Anton G.*, Jiang, Eileen*, Zhuang, Jiali, Pinello, Luca, Socci, Nicholas D.*, Koche, Richard Patrick*, Gonen, Mithat*, Villasante, Camila M.*, Armstrong, Scott Allen*, Bauer, Daniel E., Weng, Zhiping, Kentsis, Alex* * MSK affiliated

Publisher(s): Dryad

Description
Summary from Dryad:

"Abstract:
Background: Numerous human genes encode potentially active DNA transposases or recombinases, but our understanding of their functions remains limited due to shortage of methods to profile their activities on endogenous genomic substrates. Results: To enable functional analysis of human transposase-derived genes, we combined forward chemical genetic hypoxanthine-guanine phosphoribosyltransferase 1 (HPRT1) screening with massively parallel paired-end DNA sequencing and structural variant genome assembly and analysis. Here, we report the HPRT1 mutational spectrum induced by the human transposase PGBD5, including PGBD5-specific signal sequences (PSS) that serve as potential genomic rearrangement substrates. Conclusions: The discovered PSS motifs and high-throughput forward chemical genomic screening approach should prove useful for the elucidation of endogenous genome remodeling activities of PGBD5 and other domesticated human DNA transposases and recombinases."
Subject of Study
Human transposase PGBD5
Subject(s)
Genetic Testing
Genomics
Sequence Analysis, DNA
Transposases
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DOI
10.5061/dryad.t748p
Associated Publications
Henssen A, Jiang E, Zhuang J, Pinello L, Socci N, Koche R, Gonen M, Villasante C, Armstrong S, Bauer D, Weng Z, Kentsis A. Forward genetic screen of human transposase genomic rearrangements. BMC Genomics. 2016; 17(1):548.
Dataset Format(s)
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Dataset Size
313,223 KB
Data Catalog Record Updated
2023-09-27