Search Tips

Data from: Genomic DNA transposition induced by human PGBD5

UID: 10852

Author(s): Henssen, Anton G.*, Henaff, Elizabeth, Jiang, Eileen*, Eisenberg, Amy R.*, Carson, Julianne R.*, Villasante, Camila M.*, Ray, Mondira*, Still, Eric*, Burns, Melissa, Gandara, Jorge, Feschotte, Cedric, Mason, Christopher E, Kentsis, Alex* * MSK affiliated

Publisher(s): Dryad

Description
Summary from Dryad:

"Abstract:
Transposons are mobile genetic elements that are found in nearly all organisms, including humans. Mobilization of DNA transposons by transposase enzymes can cause genomic rearrangements, but our knowledge of human genes derived from transposases is limited. In this study, we find that the protein encoded by human PGBD5, the most evolutionarily conserved transposable element-derived gene in vertebrates, can induce stereotypical cut-and-paste DNA transposition in human cells. Genomic integration activity of PGBD5 requires distinct aspartic acid residues in its transposase domain, and specific DNA sequences containing inverted terminal repeats with similarity to piggyBac transposons. DNA transposition catalyzed by PGBD5 in human cells occurs genome-wide, with precise transposon excision and preference for insertion at TTAA sites. The apparent conservation of DNA transposition activity by PGBD5 suggests that genomic remodeling contributes to its biological function."
Subject of Study
Homo sapiens
Human transposase PGBD5
Subject(s)
Recombination, Genetic
Transposases
Access via Dryad

Access Restrictions
Free to All
Access Instructions
Download from Dryad to access
DOI
10.5061/dryad.b2hc1
Associated Publications
Henssen A, Henaff E, Jiang E, Eisenberg A, Carson J, Villasante C, Ray M, Still E, Burns M, Gandara J, Feschotte C, Mason C, Kentsis A. Genomic DNA transposition induced by human PGBD5. eLife. 2015; 4:e10565.
Dataset Format(s)
Microsoft Excel, BAM, FA
Dataset Size
873,744 KB (compressed)
Data Catalog Record Updated
2023-09-27