scRNA-seq to study features of CD4conv and Treg cells in spleen and bone marrow of mice

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UID: 10879

Author(s): Elias, Schlomo*, Schizas, Michail*, Rudensky, Alexander Y.* * MSK affiliated

Description: Summary from GEO:

"The aim of the study was to compare gene expression between Treg cells in the bone marrow and the spleen."

Overall design from GEO:

"2 male Foxp3-DTR-GFP were used for the study. Single cells suspension from BM and spleen were prepared, then enriched for CD4 by magneic beads and sorted by gating on CD4+TCRb+. GFP was used to differentiate between Tregs and CD4conv. CD4conv or Tregs cells from the same tissue were pooled from the two mice and hashtagged (total of 4 groups: Tregs BM/Tregs spleen/CD4 conv BM/CD4 conv spleen). The mice were not treated by DT."

Subject of Study

Subject(s)

Access via GEO

Accession #: GSE202888

Access via BioProject

Accession #: PRJNA837594
Access Restrictions: Free to All
Access Instructions: The NCBI Gene Expression Omnibus and BioProject databases provide open access to these files.
Associated Publications: Elias S, Sharma R, Schizas M, Valdez I, Rampersaud S, Park S, Gonzalez-Figueroa P, Li Q, Hoyos B, Rudensky A. CXCR4(+) Treg cells control serum IgM levels and natural IgM autoantibody production by B-1 cells in the bone marrow. Journal of Experimental Medicine. 2022; 219(7):e20220047.
Equipment Used: Illumina HiSeq 4000
High throughput gene sequencing system
Dataset Format(s): Microsoft Excel, TSV, TAR, TXT
Dataset Size: 40.9 MB

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