Regulatory logic of endogenous RNAi in silencing de novo genomic conflicts
UID: 10892
- Description
- Summary from GEO:
"Although the biological utilities of endogenous RNAi (endo-RNAi) have been largely elusive, recent studies reveal its critical role in the non-model fruitfly Drosophila simulans to suppress selfish genes, whose unchecked activities can severely impair spermatogenesis. In particular, hairpin RNA (hpRNA) loci generate endo-siRNAs that suppress evolutionary novel, X-linked, meiotic drive loci. The consequences of deleting even a single hpRNA (Nmy) in males are profound, as such individuals are nearly incapable of siring male progeny. Here, comparative genomic analyses of D. simulans and D. melanogaster mutants of the core RNAi factor dcr-2 reveal a substantially expanded network of recently-emerged hpRNA-target interactions in the former species. The de novo hpRNA regulatory network in D. simulans bears compelling signatures of sex chromosome conflict and provides insight into molecular strategies that underlie hpRNA emergence. In particular, our data support the existence of ongoing rapid evolution of Nmy/Dox-related networks, recurrent targeting of testis HMG Box loci by hpRNAs, and connections to transposons. Importantly, the impact of the endo-RNAi network on gene expression flips the convention for regulatory networks, since we observe strong derepression of targets of the youngest hpRNAs, but only mild effects on the targets of the oldest hpRNAs. These data suggest that endo-RNAi are especially critical during incipient stages of intrinsic sex chromosome conflicts, and that continual cycles of distortion and resolution may contribute to speciation."
Overall design from GEO:
"Comparative analysis of testis transcriptomic data (RNA-seq and small RNA) between wildtype and core RNAi factor mutant dcr2 in two closely related Drosophila species namely, D. melanogaster and D. simulans."
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Access via GEO
Accession #: GSE230111Access via BioProject
Accession #: PRJNA957612 - Access Restrictions
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Free to All
- Access Instructions
- The NCBI Gene Expression Omnibus and BioProject databases provides open access to these files.
- Associated Publications
- Equipment Used
- Dataset Format(s)
- TAR, BW
- Dataset Size
- 2.5 GB
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