Gene expression profile at single nucleus level for tumor associated macrophage (TAMs) nuclei sorted from tumor of control-PyMT and S100a8-creRosa26-LSL-MYCPyMT (MYC-PyMT) mice treated with low protein diet
UID: 10910
- Description
- Summary from GEO:
"Nuclei of TAMs were sorted for single nucleus RNA sequencing from control-PyMT and MYC-PyMT tumor treated with low protein diet, and compare their transcriptome differences."
Overall design from GEO:
"7-week-old Control-PyMT and MYC-PyMT mice were lethally irradiated and reconstituted with 3 million total bone marrow cells from Fcgr1-cre-Rosa26LSL-H2B-mCherry/+ mice as described previously. Two weeks later, mice were switched to a low protein diet. 6-week post dietary switch, mice were sacrificed, and tumors were collected for immune cell isolation. TAMs were sorted based on their cell surface markers (DAPI-CD45+SiglecF-Ly6G-B220-Ly6C-F4/80+Vcam+). Nuclei were isolated with an EZ PREP kit (Sigma, Cat #NUC-101) according to the manufacturer's protocol, and intact nuclei without cell contamination were further sorted by fluorescent markers (mCherry+CD11b-SYTO9+) for downstream processes."
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Access via GEO
Accession #: GSE230245Access via BioProject
Accession #: PRJNA958117 - Access Restrictions
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Free to All
- Access Instructions
- The NCBI Gene Expression Omnibus and BioProject databases provides open access to these files.
- Associated Publications
- Equipment Used
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Illumina NovaSeq 6000
- Dataset Format(s)
- TSV, MTX
- Dataset Size
- 10.3 KB (TSV), 284.1 (TSV), 11 MB (MTX)
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