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Overlapping activities of ELAV/Hu family RNA binding proteins specify the extended neuronal 3' UTR landscape in Drosophila [S2 3-seq]

UID: 10915

Description
Summary from GEO:

"The goals of this study are to establish a cell line based analysis platform to unbiasedly evaluate the capabilities of Elav/Hu family RNA binding proteins in regulating neural specific 3' UTR extension; and perform quantitative analysis of global 3' UTR extension regulated by individual Elav/Hu family RNA binding protein. We reported that in non-neural tissue context such as S2R+ cells, with the overexpression of wildtype Elav, Rbp9 and Fne, we observed global 3' UTR extensions for hundreds of genes, whose 3' UTR extension corespond with those detected in head; also, RNA rocognition motif mutant versions of Elav, Rbp9 and Fne loss the ability to regulate the generation of neural specific 3' UTR extension. We reported here for the first time, we show quantitative comparison between Elav/Hu family RNA binding proteins in regulating neural specific 3' UTR landscape and imply the overlapping activities of Elav/Hu family RNA binding proteins specifying neural 3' UTR landscape in Drosophila."

Overall design from GEO:

"3'-end sequencing profiles from S2R+ cells with the overexpression of wildtype, Elav, Rbp9, Fne and RRM mutant versions of Elav, Rbp9, Fne."
Subject of Study
Subject(s)
Access via GEO


Accession #: GSE155533

Access via BioProject


Accession #: PRJNA650192

Access via SRA


Accession #: SRP275491

Access Restrictions
Free to All
Access Instructions
The NCBI Gene Expression Omnibus, BioProject, and SRA databases provide open access to these files.
DOI
3' Untranslated Regions
Associated Publications
Equipment Used
Illumina HiSeq 1000
Lexogen QuantSeq 3′ mRNA-Seq Library Prep Kit
Dataset Format(s)
CSV, TAR, BW
Dataset Size
367.6 MB (TAR), 397.4 KB (CSV)
Data Catalog Record Updated
2023-10-10