Single-molecule mapping of replisome progression

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UID: 10918

Author(s): Claussin, Clémence*, Vazquez, Jacob*, Whitehouse, Iestyn* * MSK affiliated

Description: Summary from GEO:

"Replicon-seq is a method to study the progression of sister replisomes during DNA replication. This method relies excision of the full-length of replicons by the fusion of MNase to MCM4 and sequencing via Nanopore technology."

Overall design from GEO:

"MCM4 was fused to Miccrococcal nuclease ( MNase) to generate DNA double strand break at the site of replisomes. DNA ends are repaired and MinION compatible DNA adaptors are ligated. Full length molecules are sequenced. Because cells have been released from a G1 arrest in presence of BrdU, we can select for replicon reads (reads that contain BrdU) informatically using DNAscent."

Subject of Study

Subject(s)

Access via GEO

Accession #: GSE194025

Access via BioProject

Accession #: PRJNA798753
Access Restrictions: Free to All
Access Instructions: The NCBI Gene Expression Omnibus and BioProject databases provide open access to these files.
Associated Publications: Claussin C, Vazquez J, Whitehouse I. Single-molecule mapping of replisome progression. Molecular Cell. 2022; 82(7):1372-1382.e4.
Equipment Used: Nanopore Technology MinION
Portable device for DNA and RNA sequencing
Dataset Format(s): TAR, BED
Dataset Size: 259 MB

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