ChIP-seq on INTS11-degron cells

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UID: 10921

Author(s): Wang, Hua* * MSK affiliated

Description: Summary from GEO:

"Here, we showed that acute lost H3K4me3 induces rapid reduction in global transcription with progressively increase in magnitude over the time-course cooccurrence with lost all H3K4 methylations. To further determine the effects of COMPASS subunits degradation on H3K4 methylations occupancies genome-wide, we performed time-course spike-in chromatin immunoprecipitation and sequencing (ChIP-seq, also known as ChIP-Rx) analysis in both degron systems."

Overall design from GEO:

"INTS11-FKBP dTAG degron tag into the 3’ end of both alleles of endogenous Ints11 locus of the E14 mESC cell line."

Subject of Study

Subject(s)

Access via GEO

Accession #: GSE230123

Access via BioProject

Accession #: PRJNA957697
Access Restrictions: Free to All
Access Instructions: The NCBI Gene Expression Omnibus and BioProject databases provide open access to these files.
Associated Publications: Wang H, Fan Z, Shliaha P, Miele M, Hendrickson R, Jiang X, Helin K. H3K4me3 regulates RNA polymerase II promoter-proximal pause-release. Nature. 2023; 615(7951):339-348.
Equipment Used: Illumina NextSeq 550
Sequencing and array capabilities for transcriptome, targeted resequencing and genotype sequencing
Dataset Format(s): TAR, BW
Dataset Size: 270.3 MB

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