TT-seq on COMPASS-degron cells

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UID: 10927

Author(s): Wang, Hua* * MSK affiliated

Description: Summary from GEO:

"Here, we use elongation velocity analysis showed that acute loss H3K4me3 caused a general transcriptome-wide attenuation of elongation velocity in cells."

Overall design from GEO:

"In order to examine and monitor the effects of H3K4me3 on newly synthesized RNA rates genome-wide from actively transcribing RNAPII, we performed the modified transient transcriptome sequencing (TTchem-seq) and DRB/TTchem-seq with spike-in strategy on degron cells."

Subject of Study

Subject(s)

Access via GEO

Accession #: GSE181712

Access via BioProject

Accession #: PRJNA753083

Access via SRA

Accession #: SRP331692
Access Restrictions: Free to All
Access Instructions: The NCBI Gene Expression Omnibus, BioProject, and SRA databases provide open access to these files.
Associated Publications: Wang H, Fan Z, Shliaha P, Miele M, Hendrickson R, Jiang X, Helin K. H3K4me3 regulates RNA polymerase II promoter-proximal pause-release. Nature. 2023; 615(7951):339-348.
Equipment Used: Illumina NextSeq 550
Sequencing and array capabilities for transcriptome, targeted resequencing and genotype sequencing
Dataset Format(s): TAR, BW
Dataset Size: 810.4 MB

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