Transcriptome of mouse leptomeningeal fibroblasts

Default Boolean Operator: Terms in a search string will be connected by the AND operator, EXAMPLE: searching genomic melanoma will produce the same results as genomic AND melanoma .
AND, OR, NOT: Combine terms in a search with the boolean operators AND (to add specificity), OR (to broaden), or NOT (to eliminate a term). Operators must be capitalized (all other search terms are not case-sensitive).
Wild Card: Add wild card symbols * (open-ended) and ? (single character) to truncate terms. EXAMPLE: searching gen* shows results for 'gene', 'genetic', 'genomic, etc. Searching gene? returns results for 'gene' or 'genes' but not for 'genome'.
Phrase Searching: Enclose search in quotation marks "keyword or search term" to search for an exact phrase. EXAMPLE: "transcription factors" returns results for records where transcription is immediately followed by the word factors .
Diacritics: Words containing diacritics translate those characters to non-diacritic form. EXAMPLE: massague returns results for Massague&#769 .
Filter by: Located on the homepage (left panel), use filters to search for records or narrow the search. From search results page, filters can be removed by clicking on the term at the top of the page.

UID: 10972

Author(s): Remsik, Jan*, Saadeh, Fadi*, Boire, Adrienne* * MSK affiliated

Description: Summary from GEO:

"Meninges, or the membranous coverings of the brain and spinal cord, play host to dozens of morbid pathologies. In this study we provide a method to isolate the leptomeningeal cell layer, identify leptomeninges in histologic slides, and maintain leptomeningeal fibroblasts in in vitro culture. Using an array of transcriptomic, histological, and cytometric analyses, we identified ICAM1 and SLC38A2 as two novel markers of leptomeningeal cells in vivo and in vitro. Our results confirm the fibroblastoid nature of leptomeningeal cells and their ability to form a sheet-like layer that covers the brain and spine parenchyma. These findings will enable researchers in central nervous system barriers to describe leptomeningeal cell functions in health and disease."

Overall design from GEO:

"Mouse leptomeningeal cells were enriched using FACS and their transcriptome was assessed with bulk RNA-seq."

Subject of Study

Subject(s)

Access via GEO

Accession #: GSE150419

Access via BioProject

Accession #: PRJNA632007

Access via SRA

Accession #: SRP261321
Access Restrictions: Free to All
Access Instructions: The NCBI Gene Expression Omnibus, BioProject, and SRA databases provide open access to these files.
Associated Publications: Remsik J, Saadeh F, Tong X, Li M, Snyder J, Bale T, Wu J, Derderian C, Guber D, Chi Y, Murali R, Boire A. Characterization, isolation, and in vitro culture of leptomeningeal fibroblasts. Journal of Neuroimmunology. 2021; 361:577727.
Equipment Used: Illumina HiSeq 4000
High throughput gene sequencing system
Dataset Format(s): TXT
Dataset Size: 466.5 KB

Do you have or know of a dataset that should be added to the catalog? Let us know!