Transcriptional profiling of beta cell specific CD8 T cells from non-obese diabetic mice

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UID: 10981

Author(s): Schietinger, Andrea*, Betel, Doron*, Zumbo, Paul, Dündar, Friederike, Gearty, Sofia Elena Vaccarino* * MSK affiliated

Description: Summary from GEO:

"CD8 T cells specific for beta cell antigen islet-specific glucose-6-phosphatase catalytic subunit-related protein (IGRP) were identified and isolated with NRP-V7 tetramers from the pancreatic draining lymph node and pancreas of non-obese diabetic (NOD) mice and used for single-cell RNA-seq and VDJ-seq."

Overall design from GEO:

"Activated CD8+NRP-V7+ T cells were sorted from the pancreatic lymph node and pancreas of non-obese diabetic mice using hashtags to identify individual mice and feature barcoding antibodies against CD44, CD127, CD62L, CD73, PD1, CD38, and CD39."

Subject of Study

Subject(s)

Access via GEO

Accession #: GSE151652

Access via BioProject

Accession #: PRJNA636767

Access via SRA

Accession #: SRP265625
Access Restrictions: Free to All
Access Instructions: The NCBI Gene Expression Omnibus, BioProject, and SRA databases provide open access to these files.
Associated Publications: Gearty S, Dündar F, Zumbo P, Espinosa-Carrasco G, Shakiba M, Sanchez-Rivera F, Socci N, Trivedi P, Lowe S, Lauer P, Mohibullah N, Viale A, DiLorenzo T, Betel D, Schietinger A. An autoimmune stem-like CD8 T cell population drives type 1 diabetes. Nature. 2022; 602(7895):156-161.
Equipment Used: Illumina HiSeq 4000
High throughput gene sequencing system
Dataset Format(s): CSV, TSV, TAR, MTX, TXT, RDS
Dataset Size: 118 MB (TAR), 34.1 MB (RDS), 409.3 KB (RDS)

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