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Eomes identifies thymic precursors of self-specific memory-phenotype CD8+ T cells

UID: 11001

Author(s): Miller, Christine H., Klawon, David E., Zeng, Sharon, Lee, Victoria, Socci, Nicholas D.*, Savage, Peter A. * MSK affiliated

Description
Summary from GEO:

"Unprimed mice harbor a substantial population of "memory-phenotype" CD8+ T cells (CD8-MP cells) that exhibit hallmarks of activation and innate-like functional properties. Due to the lack of faithful markers to distinguish CD8-MP cells from bona fide CD8+ memory T cells, the developmental origins and antigen specificities of CD8-MP cells remain incompletely defined. Using deep T cell receptor (TCR) sequencing, we found that the TCRs expressed by CD8-MP cells are highly recurrent and distinct from the TCRs expressed by naive-phenotype CD8+ T cells. CD8-MP clones exhibited reactivity to widely expressed self-ligands. T cell precursors expressing CD8-MP TCRs upregulated the transcription factor Eomes during maturation in the thymus, prior to induction of the full memory phenotype, suggestive of a unique program triggered by recognition of self-ligands. Moreover, CD8-MP cells infiltrate oncogene-driven prostate tumors and express high densities of PD-1, suggesting a potential role in anti-tumor immunity and response to immunotherapy."

Overall design from GEO:

"TCRalpha chains were sequenced from FACS-purified CD8-Naïve (CD8+ CD44lo CD122neg) and CD8-MP (CD8+ CD44hi CD122+) T cells from the pooled spleen and lymph nodes of mice expressing a fixed transgenic TCRbeta. Cells were purified from five different 9-week-old male mice on the C57BL/6J (B6) background. In addition, TCRalpha chains were sequenced from FACS-purified CD8+ T cells from the prostate tumors of five 27-week-old TRAMP+/- males expressing the fixed TCRbeta chain."
Subject of Study
Mus musculus
Subject(s)
CD8-Positive T-Lymphocytes
Prostatic Neoplasms
Receptors, Antigen, T-Cell
Transcription Factors
Access via GEO


Accession #: GSE145365

Access via BioProject


Accession #: PRJNA606961

Access via SRA


Accession #: SRP249654

Access Restrictions
Free to All
Access Instructions
The NCBI Gene Expression Omnibus, BioProject, and SRA databases provide open access to these files.
Associated Publications
Miller C, Klawon D, Zeng S, Lee V, Socci N, Savage P. Eomes identifies thymic precursors of self-specific memory-phenotype CD8(+) T cells. Nature Immunology. 2020; 21(5):567-577.
Equipment Used
454 GS
Dataset Format(s)
CSV, TAR
Dataset Size
620 KB
Data Catalog Record Updated
2023-11-03