Transfer of antigen target into CAR T cells via trogocytosis

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UID: 11084

Author(s): Li, Zhuoning*, Sadelain, Michel W. J.* * MSK affiliated

Description: Description from PRIDE:

"To characterize transfer of molecules from target cells into CAR T cells via trogocytosis we cultured NALM-6 leukemia cell line expressing a CD19-mCherry fusion protein with CAR T cells. NALM6-CD19-mCherry were loaded with heavy amino acid and cocultured with CAR T cells for 1 hour. CAR T cells were next sorted into two fractions, mCherry-positive (TrogPos), and -negative (TrogNeg). Proteomics analysis revealed the presence of targeted antigen (CD19) in the TrogPos only."

Subject of Study

Subject(s)

Access via PRIDE

Accession #: PXD012833
Access Restrictions: Free to All
Access Instructions: All public datasets available in PRIDE Archive in the repository can be accessed via the website by FTP link, Web Service (for programmatic access), and the PRIDE Inspector stand-alone tool.
Associated Publications: Hamieh M, Dobrin A, Cabriolu A, van der Stegen S, Giavridis T, Mansilla-Soto J, Eyquem J, Zhao Z, Whitlock B, Miele M, Li Z, Cunanan K, Huse M, Hendrickson R, Wang X, Rivière I, Sadelain M. CAR T cell trogocytosis and cooperative killing regulate tumour antigen escape. Nature. 2019; 568(7750):112-116.
Equipment Used: Q Exactive
Mass Spectrometer

SILAC Protein Quantitation Kit (Trypsin), RPMI 1640
Used for the specific analysis of protein expression by mass spectrometry using stable isotope labeling with amino acids in cell culture (SILAC)
Software Used: MaxQuant
A quantitative proteomics software package designed for analyzing large mass-spectrometric data sets. It is specifically aimed at high-resolution MS data.
Dataset Format(s): Microsoft Excel, RAW
Dataset Size: 51 GB

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