Accumulated ion monitoring for improved sensitivity

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UID: 11089

Author(s): Kentsis, Alex* * MSK affiliated

Description: This work aimed to improve sensitivity of targeted detection by orbitrap mass spectrometers. Co-isolation of contaminant ions was identified as the major factor limiting sensitivity, and LOD of both PRM and accumulated precursor ion scanning (AIM) was improved by increased chromatographic resolution.

Subject of Study

Subject(s)

Access via PRIDE

Accession #: PXD006236
Access Restrictions: Free to All
Access Instructions: All public datasets available in PRIDE Archive in the repository can be accessed via the website by FTP link, Web Service (for programmatic access), and the PRIDE Inspector stand-alone tool.
Associated Publications: Cifani P, Kentsis A. High sensitivity quantitative proteomics using automated multidimensional nano-flow chromatography and accumulated ion monitoring on quadrupole-orbitrap-linear ion trap mass spectrometer. Molecular & Cellular Proteomics. 2017; 16(11):2006-2016.
Equipment Used: Orbitrap Fusion Lumos
Software Used: Skyline
Creates and refines targeted methods for large-scale quantitative mass spectrometry studies in life sciences

Thermo Xcalibur™
method setup, data acquisition, data processing, and data acquisition and processing integration
Dataset Format(s): RAW, OTHER

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