Synthetic fluoro peptides in neat solvent and complex proteome background

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UID: 11104

Author(s): Kentsis, Alex* * MSK affiliated

Description: This dataset was generated to model discovery of uncommon and non-canonical PTM by agnostic analysis. Synthetic DFSAFILVEFCR peptides were obtained in three chemoforms, all with carbamidomethylated C12: 1) with unmodified phenylalanines, 2) with fluoro-F2, and 3) with fluoro-F11. Peptide composition and chemical modifications were confirmed using mass spectrometry. Peptides were analyzed in neat solvent for sequence and modification confirmation, and in the context of AML cell tryptic proteome.

Subject of Study

Subject(s)

OncoTree Cancer Type(s)

Acute Myeloid Leukemia

Access via PRIDE

Accession #: PXD019827
Access Restrictions: Free to All
Access Instructions: All public datasets available in PRIDE Archive in the repository can be accessed via the website by FTP link, Web Service (for programmatic access), and the PRIDE Inspector stand-alone tool.
Associated Publications: Cifani P, Li Z, Luo D, Grivainis M, Intlekofer A, Fenyö D, Kentsis A. Discovery of protein modifications using differential tandem mass spectrometry proteomics. Journal of Proteome Research. 2021; 20(4):1835-1848.
Equipment Used: Orbitrap Fusion Lumos
Software Used: Byonic
Full MS/MS search engine for comprehensive peptide and protein identification
Dataset Format(s): Microsoft Excel, RAW
Dataset Size: 800 MB

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