Validation of itaconate modification on model proteins

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UID: 11106

Author(s): Kentsis, Alex* * MSK affiliated

Description: This dataset was generated to confirm that +130 and +146 Da adducts observed in LPS-stimulated macrophages were produced by the itaconate metabolite. To this end, model proteins (bovine serum albumin and human KEAP1) were reacted in vitro with itaconate, and the correspondent adducts were analyzed by LC-MSMS

Subject of Study

Subject(s)

Access via PRIDE

Accession #: PXD019858
Access Restrictions: Free to All
Access Instructions: All public datasets available in PRIDE Archive in the repository can be accessed via the website by FTP link, Web Service (for programmatic access), and the PRIDE Inspector stand-alone tool.
Associated Publications: Cifani P, Li Z, Luo D, Grivainis M, Intlekofer A, Fenyö D, Kentsis A. Discovery of protein modifications using differential tandem mass spectrometry proteomics. Journal of Proteome Research. 2021; 20(4):1835-1848.
Equipment Used: Q Exactive HF
Mass Spectrometer
Software Used: SAMPEI
open-source algorithm designed for the discovery of functional protein and peptide signaling modifications

X! Tandem
software that can match tandem mass spectra with peptide sequences, in a process that has come to be known as protein identification.
Dataset Format(s): RAW, TXT
Dataset Size: 8 GB

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