Proteins associated with Cdc48A identified by LC-MS\MS data

UID: 11110

Author(s): Dantuluri, Swathi*, Maupin-Furlow, Julie * MSK affiliated

Description: His6-Cdc48a and associated proteins purified from H. volcanii. A) SDS- PAGE of proteins purified by Ni2+ -chromatography. H. volcanii carrying empty vector control (H1209-pJAM202c, lane 1) and ectopically expressing His6-Cdc48a (H1209- pJAM1409, lane 2). Red bars indicate regions of gel excised. B) Proteins identified in gel slices by LC-MS/MS analysis. Criteria for protein inclusion: FDR < 0.01%, normalized total spectra minus control > 50, protein threshold > 99% and 2 peptide minimum. Based on spectral counting, Cdc48a and DUF111 were most abundant in regions (a) and (b), respectively, as indicated. DHS1, deoxyhypusine synthase. DUF111, Cdc48a gene neighbor of unknown function. TnaA, tryptophanase; Rps3, ribosomal protein S3; n.d., not detected.

Subject of Study

Subject(s)

Access via PRIDE

Accession #: PXD019906
Access Restrictions: Free to All
Access Instructions: All public datasets available in PRIDE Archive in the repository can be accessed via the website by FTP link, Web Service (for programmatic access), and the PRIDE Inspector stand-alone tool.
Associated Publications: Jia H, Dantuluri S, Margulies S, Smith V, Lever R, Allers T, Koh J, Chen S, Maupin-Furlow JA. RecJ3/4-aRNase J form a Ubl-associated nuclease complex functioning in survival against DNA damage in Haloferax volcanii. mBio. 2023 Aug 31;14(4):e0085223. Epub 2023 Jul 17. PMID: 37458473; PMCID: PMC10470531.
Equipment Used: LTQ Orbitrap
Hybrid FT Mass Spectrometer
Software Used: Mascot
For identification, characterisation and quantitation of proteins using mass spectrometry data.

Scaffold
DDA and DIA proteomics and small molecule mass spectrometry software solutions for handling large-scale, data-rich, biological identification and quantitative experiments.
Dataset Format(s): RAW, MGF
Dataset Size: 6.4 GB

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