RNase J, RecJ3, RNase J, RecJ3, Cdc48a and Cas7 co-purify with RecJ4-StrepII in the presence of γATP. and Cas7 co-purify with RecJ4-StrepII in the presence of γATP.

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UID: 11112

Author(s): Dantuluri, Swathi*, Maupin-Furlow, Julie * MSK affiliated

Description: Detecting core complex formation for RecJ3/4 and RNase J form a core complex.

Subject of Study

Subject(s)

Access via PRIDE

Accession #: PXD019932
Access Restrictions: Free to All
Access Instructions: All public datasets available in PRIDE Archive in the repository can be accessed via the website by FTP link, Web Service (for programmatic access), and the PRIDE Inspector stand-alone tool.
Associated Publications: Jia H, Dantuluri S, Margulies S, Smith V, Lever R, Allers T, Koh J, Chen S, Maupin-Furlow JA. RecJ3/4-aRNase J form a Ubl-associated nuclease complex functioning in survival against DNA damage in Haloferax volcanii. mBio. 2023 Aug 31;14(4):e0085223. Epub 2023 Jul 17. PMID: 37458473; PMCID: PMC10470531.
Equipment Used: LTQ Orbitrap
Hybrid FT Mass Spectrometer
Software Used: Mascot
For identification, characterisation and quantitation of proteins using mass spectrometry data.

Scaffold
DDA and DIA proteomics and small molecule mass spectrometry software solutions for handling large-scale, data-rich, biological identification and quantitative experiments.
Dataset Format(s): RAW, MGF
Dataset Size: 3 GB