Analysis of ERalpha PPI in breast cancer cells by LC-MS/MS

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UID: 11117

Author(s): Strillacci, Antonio*, Bromberg, Jacqueline* * MSK affiliated

Description: Identification of protein-protein interaction (PPI) of a novel variant of estrogen receptor alpha in breast cancer cells

Subject of Study

Subject(s)

Access via PRIDE

Accession #: PXD027088
Access Restrictions: Free to All
Access Instructions: All public datasets available in PRIDE Archive in the repository can be accessed via the website by FTP link, Web Service (for programmatic access), and the PRIDE Inspector stand-alone tool.
Associated Publications: Strillacci A, Sansone P, Rajasekhar V, Turkekul M, Boyko V, Meng F, Houck-Loomis B, Brown D, Berger M, Hendrickson R, Chang Q, de Stanchina E, Pareja F, Reis-Filho J, Segu Rajappachetty R, Del Priore I, Liu B, Cai Y, Penson A, Mastroleo C, Berishaj M, Borsetti F, Spisni E, Lyden D, Chandarlapaty S, Bromberg J. ERα-LBD, an isoform of estrogen receptor alpha, promotes breast cancer proliferation and endocrine resistance. npj Breast Cancer. 2022; 8:96.
Equipment Used: nanoACQUITY UPLC
This system leverages nanoscale UPLC separations and high-resolution MSE mass spectrometry to answer important questions about biotherapeutic protein dynamics, conformation, and interactions

Q Exactive
Mass Spectrometer
Software Used: Mascot
For identification, characterisation and quantitation of proteins using mass spectrometry data.

Scaffold
DDA and DIA proteomics and small molecule mass spectrometry software solutions for handling large-scale, data-rich, biological identification and quantitative experiments.
Dataset Format(s): RAW
Dataset Size: 4.2 GB

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