Oxidized thioredoxin-1 restrains the NLRP1 inflammasome

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UID: 11123

Author(s): Ball, Daniel*, Bachovchin, Daniel* * MSK affiliated

Description: To find cellular protein binding partners of the NLRP1 inflammasome, FLAG-tagged full length NLRP1 (or a GFP-FLAG protein control) was ectopically expressed in HEK 293T cells and affinity purified from cell lysates (Note: the NLRP1-FLAG-containing lysate samples were affinity purified in the presence or absence of excess proline) before proteins were reduced, alkylated, and trypsinized before TMT labelling and pooling of the differentially tested lysates to give three conditions (1. NLRP1-FLAG; 2. GFP-FLAG; 3. NLRP1-FLAG+proline) in duplicate. These data represent the results following sample and data processing.

Subject of Study

Subject(s)

Access via PRIDE

Accession #: PXD037391
Access Restrictions: Free to All
Access Instructions: All public datasets available in PRIDE Archive in the repository can be accessed via the website by FTP link, Web Service (for programmatic access), and the PRIDE Inspector stand-alone tool.
Associated Publications: Ball D, Tsamouri L, Wang A, Huang H, Warren C, Wang Q, Edmondson I, Griswold A, Rao S, Johnson D, Bachovchin D. Oxidized thioredoxin-1 restrains the NLRP1 inflammasome. Science Immunology. 2022; 7(77):eabm7200.
Equipment Used: Orbitrap Fusion Lumos
Software Used: Proteome Discoverer
For the identification and quantification of proteins in complex biological samples
Dataset Format(s): Microsoft Excel, RAW
Dataset Size: 2.1 GB

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