HEK293T cell degradome (pulse chase) time course (5h, 10, 15h) analysis upon Torin1 treatment. Related to Supplementary Table 3 from An et al (2020)

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UID: 11129

Author(s): Ordureau, Alban Simon Georges*, Harper, Wade J. * MSK affiliated

Description: HEK293T Azidohomoalanine (AHA) Pulse Chase (degradome) time course experiment, were cells from various genetic background (WT, ATG7-/-, RB1CC1-/-) are treated or not with Torin1 for 5h, 10h or 15h. Samples are Biotin-click, and streptavidin enriched, digested and labeled with TMTpro 16plex.

Subject of Study

Subject(s)

Access via PRIDE

Accession #: PXD018158
Access Restrictions: Free to All
Access Instructions: All public datasets available in PRIDE Archive in the repository can be accessed via the website by FTP link, Web Service (for programmatic access), and the PRIDE Inspector stand-alone tool.
Associated Publications: An H, Ordureau A, Körner M, Paulo JA, Harper JW. Systematic quantitative analysis of ribosome inventory during nutrient stress. Nature. 2020 Jul;583(7815):303-309. Epub 2020 Jul 1. PMID: 32612236; PMCID: PMC7351614.
Equipment Used: Orbitrap Fusion Lumos
Dataset Format(s): RAW, MSF
Dataset Size: 2.5 GB

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