HEK293T cell proteome remodeling upon Torin1 treatment or amino acid withdraw in WT and RPS3-Halo knock-in cells with or without NUFIP1 deletion. Related to Supplementary Table 5 from An et al (2020)

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UID: 11131

Author(s): Ordureau, Alban Simon Georges*, Harper, Wade J. * MSK affiliated

Description: HEK293T proteome (single shot) with knock-in or not for RPS3-Halo and Knock out or not for NUFIP1. Cells were treated or not with Torin1 or deprived of amino acids.

Subject of Study

Subject(s)

Access via PRIDE

Accession #: PXD017851
Access Restrictions: Free to All
Access Instructions: All public datasets available in PRIDE Archive in the repository can be accessed via the website by FTP link, Web Service (for programmatic access), and the PRIDE Inspector stand-alone tool.
Associated Publications: An H, Ordureau A, Körner M, Paulo JA, Harper JW. Systematic quantitative analysis of ribosome inventory during nutrient stress. Nature. 2020 Jul;583(7815):303-309. Epub 2020 Jul 1. PMID: 32612236; PMCID: PMC7351614.
Equipment Used: Orbitrap Fusion Lumos
Software Used: Percolator
a software for postprocessing of shotgun proteomics data

SEQUEST®
A tandem mass spectrometry database search program that correlates tandem mass spectra of peptides against peptide sequences from a sequence database.
Dataset Format(s): RAW, MSF
Dataset Size: 10.5 GB

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