Spo11-oligo mapping of double-strand breaks in spo11 mutants

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UID: 11172

Author(s): Tischfield, Sam E.*, Keeney, Scott * MSK affiliated

Description: Summary from GEO:

"The Spo11-generated double-strand breaks (DSBs) that initiate meiotic recombination are non-randomly distributed across the genome. Here, we use Spo11-oligonucleotide complexes to map the distribution of meiotic DSBs in a spo11 mutant strain of Saccharomyces cerevisiae."

Overall design from GEO:

"4 samples total: two biological replicate Spo11-oligo maps of S. cerevisiae SK1 spo11-F260A and wildtype."

Subject of Study

Subject(s)

Access via GEO

Accession #: GSE150314

Access via BioProject

Accession #: PRJNA631755

Access via SRA

Accession #: SRP261136
Access Restrictions: Free to All
Access Instructions: The NCBI Gene Expression Omnibus. BioProject, and SRA databases provide open access to these files.
Associated Publications: Claeys Bouuaert C, Tischfield S, Pu S, Mimitou E, Arias-Palomo E, Berger J, Keeney S. Structural and functional characterization of the Spo11 core complex. Nature Structural and Molecular Biology. 2021; 28(1):92-102.
Equipment Used: Illumina HiSeq 2500
High throughput sequencing system
Dataset Format(s): TAR, WIG
Dataset Size: 11.8 MB

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