Effects of Ars2 or DGCR8 siRNA on gene expression

Default Boolean Operator: Terms in a search string will be connected by the AND operator, EXAMPLE: searching genomic melanoma will produce the same results as genomic AND melanoma .
AND, OR, NOT: Combine terms in a search with the boolean operators AND (to add specificity), OR (to broaden), or NOT (to eliminate a term). Operators must be capitalized (all other search terms are not case-sensitive).
Wild Card: Add wild card symbols * (open-ended) and ? (single character) to truncate terms. EXAMPLE: searching gen* shows results for 'gene', 'genetic', 'genomic, etc. Searching gene? returns results for 'gene' or 'genes' but not for 'genome'.
Phrase Searching: Enclose search in quotation marks "keyword or search term" to search for an exact phrase. EXAMPLE: "transcription factors" returns results for records where transcription is immediately followed by the word factors .
Diacritics: Words containing diacritics translate those characters to non-diacritic form. EXAMPLE: massague returns results for Massague&#769 .
Filter by: Located on the homepage (left panel), use filters to search for records or narrow the search. From search results page, filters can be removed by clicking on the term at the top of the page.

UID: 11225

Author(s): Olejniczak, Scott Henry*, Gruber, Joshua, Dreyfuss, Gideon, Thompson, Craig B.* * MSK affiliated

Description: Summary from GEO:

"Ars2 is a component of the nuclear cap-binding complex that is required for cellular proliferation and contributes to microRNA biogenesis. Arrays were performed to determine the repertoire of genes that change following knock-down of Ars2. Knock-down of DGCR8 was also performed to determine which changes in Ars2 knock-down cells resulted from defects in microRNA expression."

Overall design from GEO:

"9 samples were analyzed including: three biological replicates of control siRNA-transfected HeLa cells, HeLa cells transfected with three independent siRNAs targeting Ars2, or HeLa cells transfected with three independent siRNAs targeting DGCR8."

Subject of Study

Subject(s)

Access via GEO

Accession #: GSE34679

Access via BioProject

Accession #: PRJNA156237
Access Restrictions: Free to All
Access Instructions: The NCBI Gene Expression Omnibus and BioProject databases provides open access to these files.
Associated Publications: Gruber J, Olejniczak S, Yong J, La Rocca G, Dreyfuss G, Thompson C. Ars2 Promotes proper replication-dependent histone mRNA 3' end formation. Molecular Cell. 2012; 45(1):87-98.
Equipment Used: Affymetrix Human Exon 1.0 ST Array
Dataset Format(s): TAR, CEL, CHP
Dataset Size: 39.4 MB

Do you have or know of a dataset that should be added to the catalog? Let us know!