Effects of Ars2 or DGCR8 siRNA on microRNA expression

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UID: 11226

Author(s): Olejniczak, Scott Henry*, Gruber, Joshua, Dreyfuss, Gideon, Thompson, Craig B.* * MSK affiliated

Description: Summary from GEO:

"Ars2 is a component of the nuclear cap-binding complex that is required for cellular proliferation and contributes to microRNA biogenesis. Arrays were performed to determine the repertoire of microRNAs that change following knock-down of Ars2. Knock-down of DGCR8 was also performed to determine which microRNAs were expressed and had half-lives short enough to significantly decrease over the duration of the experiment."

Overall design from GEO:

"9 samples were analyzed including: three biological replicates of control siRNA-transfected HeLa cells, HeLa cells transfected with three independent siRNAs targeting Ars2, or HeLa cells transfected with three independent siRNAs targeting DGCR8."

Subject of Study

Subject(s)

Access via GEO

Accession #: GSE34680

Access via BioProject

Accession #: PRJNA156235
Access Restrictions: Free to All
Access Instructions: The NCBI Gene Expression Omnibus and BioProject databases provides open access to these files.
Associated Publications: Gruber J, Olejniczak S, Yong J, La Rocca G, Dreyfuss G, Thompson C. Ars2 Promotes proper replication-dependent histone mRNA 3' end formation. Molecular Cell. 2012; 45(1):87-98.
Equipment Used: Affymetrix Multispecies miRNA-1 Array
Dataset Format(s): TAR, CEL, CHP
Dataset Size: 1.8 MB

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